NF200 IHC Antibody

Description

Neurofilaments are composed of 3 neuron-specific proteins with 

apparent molecular masses of 68 kD(NFL), 125 kD(NFM), and 

200 kD(NFH) on SDS-gel electrophoresis. Genomic clones for 

the largest human neurofilament protein(NF-H) were isolated, the

 intron/exon boundaries mapped and the entire protein-coding 

regions(exons) sequenced. mutations in neurofilaments have 

been linked to some forms of Charcot-Marie-Tooth disease(CMT).

Catalog Number

IW-MA1071

Quantity

9 ml

Host

Mouse

Clone

NF-200

Isotype

Mouse IgG1

Immunogen

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

Purity

Purified by the goat anti-mouse IgG affinity chromatography

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Brain

Cellular Localization

Cytoplasmic

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Formalin fixed paraffin embedded tissue sections.

IHC-Fr: Formalin or acetone fixed frozen sections.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Lees, J. F.; Shneidman, P. S.; Skuntz, S. F.; Carden, M. J.; Lazzarini, R. A. : The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it. EMBO J. 7: 1947-1955, 1988.

2. Brownlees, J.; Ackerley, S.; Grierson, A. J.; Jacobsen, N. J. O.; Shea, K.; Anderton, B. H.; Leigh, P. N.; Shaw, C. E.; Miller, C. C. J. : Charcot-Marie-Tooth disease neurofilament mutations disrupt neurofilament assembly and axonal transport. Hum. Molec. Genet. 11: 2837-2844, 2002.