Description |
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. Vascular endothelial growth factor is a mitogen primarily for vascular endothelial cells. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. Vascular endothelial growth factor (VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo.
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Catalog Number
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IW-PA1080 |
Quantity
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9 ml |
Host
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Rabbit
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Clone
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Polyclonal
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Isotype
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IgG
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Immunogen
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A synthetic peptide corresponding to a sequence near the N-teminal end of VEGF of human origin, identical to the related rat sequences.
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Purity
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Immunogen affinity purified
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Conjugate
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Unconjugated
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Species Reactivity
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Human, mouse, rat.Not tested in other species. |
Positive Control
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Skin, Human esophagus squamous carcinoma.
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Cellular Localization
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Cytoplasmic
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Form
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Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.
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Storage
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Store at 2-8 °C. Do not freeze.
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Applications
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IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (Cat# IW-1100) is recommended. IHC-Fr: Not tested. ICC: Not tested.
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Limitations
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This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.
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Precautions
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Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.
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References
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1. Lee, C. G.; Link, H.; Baluk, P.; Homer, R. J.; Chapoval, S.; Bhandari, V.; Kang, M. J.; Cohn, L.; Kim, Y. K.; McDonald, D. M.; Elias, J. A. : Vascular endothelial growth factor (VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. Nature Med. 10: 1095-1103, 2004. 2. Gerber, H.-P.; Malik, A. K.; Solar, G. P.; Sherman, D.; Liang, X. H.; Meng, G.; Hong, K.; Marsters, J. C.; Ferrara, N. : VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Nature 417: 954-958, 2002. 3. Jin, K.; Zhu, Y.; Sun, Y.; Mao, X. O.; Xie, L.; Greenberg, D. A. : Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo. Proc. Nat. Acad. Sci. 99: 11946-11950, 2002.
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