MMP16 IHC Antibody

Description

The matrix metalloproteinase 16 (MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21¹. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17². Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms². MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.

Catalog Number

IW-PA1123

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide mappingat the C-terminal of human MMP16, identical to the related rat sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, rat.Not tested in other species.

Positive Control

Kidney

Cellular Localization

Cytoplasmic

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval improve stain on formalin fixed paraffin sections.

IHC-Fr: Formalin or acetone fixed tissue.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1.Sato, H.; Tanaka, M.; Takino, T.; Inoue, M.; Seiki, M. : Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization. Genomics 39: 412-413, 1997.

2.Takino, T.; Sato, H.; Shinagawa, A.; Seiki, M. : Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library: MT-MMPs form a unique membrane-type subclass in the MMP family. J. Biol. Chem. 270: 23013-23020, 1995.