TFPI-2 IHC Antibody

Description

Tissue factor pathway inhibitor 2, also known as TFPI2, is a human gene which is located at 7q22. It is an important regulator of the extrinsic pathway of blood coagulation through its ability to inhibit factor Xa and factor VIIa-tissue factor activity. After a 22-residue signal peptide, the mature TFPI2 protein contains 213 amino acids with 18 cysteines and 2 canonical N-linked glycosylation sites. The purified

recombinant TFPI2 strongly inhibited the amidolytic activities of trypsin and the factor VIIa-tissue factor complex.1 The latter inhibition was markedly enhanced in the presence of heparin. Mouse TFPI2 mRNA is highly expressed in developing mouse placenta, as in human.2 And there are also high transcript levels in adult mouse liver and kidney.

Catalog Number

IW-PA1248

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the Middle region of human TFPI2, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human. Not tested in other species.

Positive Control

Human mammary cancer, placenta, liver, kidney.

Cellular Localization

Cytoplasmic

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat Induced Epitope Retrieval is required on formalin fixed paraffin embedded tissue sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Sprecher, C. A.; Kisiel, W.; Mathewes, S.; Foster, D. C. : Molecular cloning, expression, and partial characterization of a second human tissue-factor-pathway inhibitor. Proc. Nat. Acad. Sci. 91: 3353-3357, 1994.

2. Miyagi, Y.; Yasumitsu, H.; Mizushima, H.; Koshikawa, N.; Matsuda, Y.; Itoh, H.; Hori, T.-A.; Aoki, I.; Misugi, K.; Miyazaki, K. : Cloning of the cDNA encoding mouse PP5/TFPI-2 and mapping of the gene to chromosome 6. DNA and Cell Biology 15: 947-954, 1996.