PCNA IHC Antibody

Description

Proliferating cell nuclear antigen(PCNA) was originally identified by immunofluorescence
as a nuclear protein whose appearance correlated with the proliferative state of the cell.
PCNA/cyclin has been localized by in situ hybridization to the short arm of human 
chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single
 copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and
DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth
factors. PCNA controls establishment of sister chromatid cohesion during S phase.

Catalog Number

IW-MA1083

Quantity

9 ml

Host

Mouse

Clone

IML-83

Isotype

Mouse IgG2a

Immunogen

Protein A fusion protein.

Purity

Purified by the goat anti-mouse IgG affinity chromatography

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Skin, Hela cells

Cellular Localization

Nuclear

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin embedded sections. IHC-Tek Epitope Retrieval Solution (IW-1100) is recommended.

IHC-Fr: Formalin fixed or acetone fixed frozen tissues.

ICC: Suitable.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Bravo, R. : Synthesis of the nuclear protein cyclin (PCNA) and its relationship with DNA replication. Exp. Cell Res. 163: 287-293, 1986.

2. Moldovan, G.-L.; Pfander, B.; Jentsch, S. : PCNA controls establishment of sister chromatid cohesion during S phase. Molec. Cell 23: 723-732, 2006.

3. Webb, G.; Parsons, P.; Chenevix-Trench, G. : Localization of the gene for human proliferating nuclear antigen/cyclin by in situ hybridization. Hum. Genet. 86: 84-86, 1990.