Description |
HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
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Catalog Number
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IW-PA1106 |
Quantity
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9 ml |
Host
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Rabbit
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Clone
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Polyclonal
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Isotype
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IgG
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Immunogen
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A synthetic peptide mapping at the middle region of human HSP60, identical to the related rat and mouse sequence.
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Purity
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Immunogen affinity purified
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Conjugate
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Unconjugated
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Species Reactivity
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Human, mouse, rat.Not tested in other species. |
Positive Control
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Human intestinal cancer, mammary cancer.
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Cellular Localization
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Cytoplasmic
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Form
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Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.
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Storage
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Store at 2-8 °C. Do not freeze.
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Applications
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IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Fr: Suitable. ICC: Suitable.
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Limitations
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This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.
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Precautions
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Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.
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References
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