Anti-YWHAE / 14-3-3 Epsilon Antibody (N-Terminus) IHC-plus LS-B3683

Product Description

Presentation: Serum, 0.09% sodium azide.

Target Species: Sheep

Host Species: Rabbit

Specificity: Recognizes the acetylated N-terminal of 14-3-3 epsilon. It is a member of the 14-3-3 family which consists of 30 kD proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G(2) checkpoint. During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene. May not react with recombinant proteins that are not N-acetylated.

Reactivity: Chicken, Sheep, Bovine, Rabbit, Mouse, Rat, Human

Usage: ELISA, IHC-P (1:400), WB (1:3000)

Synonyms: YWHAE, 14-3-3E, 14-3-3 epsilon, 14-3-3 protein epsilon, MDCR, MDS, KCIP-1

Concentration:

Volume: 50 µl

Immunogen: Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 epsilon. ( N-TERMINAL ).