Specificity: Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.
Reactivity: Mouse, Dog, Sheep, Bovine, Rat, Pig, Horse, Human
Usage: Flo, IF, IHC-P (10 g/ml), IP, WB
Synonyms:
Concentration: 1 mg/ml
Volume: 50 µl
Immunogen: Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.