Anti-Alpha Tubulin Antibody (C-Terminus, clone YL1/2) IHC-plus LS-B6777

$495.00

SKU:

LS-B6777

Product Description

Presentation: PBS, pH 7.4, 0.09% sodium azide.

Target Species: Yeast

Host Species: Rat

Specificity: Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin

Reactivity: Avian, Echinoderm, Mammal, Human, Yeast

Usage: ELISA (1:100 - 1:1000), IHC-Fr, IHC-P (5 g/ml), IP, RIA, WB

Synonyms:

Concentration: 1 mg/ml

Volume:

Immunogen: Yeast tubulin.