Polink-2 Plus Series overcomes the weaknesses of current 2-step polymer detection systems. It demonstrates ultra sensitivity and high specificity to mouse, rabbit, goat and rat antibodies. This system reduces incubation time by 10 minutes for each incubation step, saving a total of 20-30 minutes for the entire process.
Detecting RAT primary antibody on MOUSE tissue is a very difficult task in research field due to background staining issues. Polink-2 Plus HRP or AP Rat-NM Detection Kit is specially designed to solve the problem. The secondary antibody is adsorbed to mouse and human serum proteins. This technology provides excellent specificity to detect rat primary antibody (user supplied) on mouse tissue. Besides mouse tissue Polink-2 Plus Rat-NM Detection kit also can be used on human tissue as well.
Polink-2 Plus Rat-NM Detection Kit uses rat antibody enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces consistent immunostaining outcomes on archival tissues and on difficult-to-work antibodies. User may need to further dilute primary antibody due to super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore it overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible with either manual or automated systems. Staining conditions need to be optimized by user.
1. Enhancer for Rat (no cross to mouse), 2. Polymer HRP x Rat, 3. with DAB
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