Polink-2 Plus Series overcomes the weaknesses of current 2-step polymer detection systems. It demonstrates ultra sensitivity and high specificity to mouse, rabbit, goat and rat antibodies. This system reduces incubation time by 10 minutes for each incubation step, saving a total of 20-30 minutes for the entire process.
Detecting MOUSE primary antibody on RAT tissue is a very difficult task in research field due to background staining issues. Polink-2 Plus HRP or AP Mouse-NR (no cross react to rat) Detection Kit is specially designed to solve the problem. The secondary antibody is adsorbed to rat, rabbit and human serum proteins. This technology provides excellent specificity to detect mouse primary antibody (user supplied) on rat tissue. Besides rat tissue Polink-2 Plus Mouse-NR Detection kit also can be used on human tissue and rabbit tissue as well.
Polink-2 Plus Mouse-NR Detection Kit is a ultra sensitive polymer detection system. It uses mouse antibody enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces consistent immunostaining outcomes on archival tissues and on difficult-to-work antibodies. User may need to further dilute primary antibody due to super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore it overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible with either manual or automated systems. Staining conditions need to be optimized by user.
1. Enhancer for Mouse (no cross to Rat), 2. Polymer HRP x Ms, 3. with DAB
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