CD54 (ICAM-1) IHC Antibody

Description

CD54 also knows as ICAM-1. Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. ICAM1 protein is the major human rhinovirus receptor. ICAM1 gene is mapped to human chromosome 19. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).

Catalog Number

IW-PA1110

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A peptide mapping at the C-terminal region of the human ICAM-1, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human. Not tested in other species.

Positive Control

Human mammary cancer, human tonsil.

Cellular Localization

Membrane/Cytoplasmic

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Proteinase K pretreatment is required on formalin fixed paraffin sections. IHC-Tek Proteinase K Solution (Cat# IW-1101) is recommended.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Ballantyne, C. M.; Kozak, C. A.; O'Brien, W. E.; Beaudet, A. L. : Assignment of the gene for intercellular adhesion molecule-1 (Icam-1) to proximal mouse chromosome 9. Genomics 9: 547-550, 1991.

2. Greve, J. M.; Davis, G.; Meyer, A. M.; Forte, C. P.; Yost, S. C.; Marlor, C. W.; Kamarck, M. E.; McClelland, A. : The major human rhinovirus receptor is ICAM-1. Cell 56: 839-847, 1989.

3. Prieto, J.; Takei, F.; Gendelman, R.; Christenson, B.; Biberfeld, P.; Patarroyo, M. : MALA-2, mouse homologue of human adhesion molecule ICAM-1 (CD54). Europ. J. Immun. 19: 1551-1557, 1989.