SKP2 (S-phase kinase-associated protein 2) IHC Antibody

Product Description

Description

The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.

Catalog Number

IW-PA1102

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminal of human SKP2, different from the related rat and mouse sequence by three amino acids.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat.Not tested in other species.

Positive Control

Human intestinal cancer, Hela cells.

Cellular Localization

Cytoplasmic/membrane

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval enhance stain on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Acetone fixed cells.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Kitagawa, M.; Lee, S. H.; McCormick, F. : Skp2 suppresses p53-dependent apoptosis by inhibiting p300. Molec. Cell 29: 217-231, 2008.
2. Latres, E.; Chiarle, R.; Schulman, B. A.; Pavletich, N. P.; Pellicer, A.; Inghirami, G.; Pagano, M. : Role of the F-box protein Skp2 in lymphomagenesis. Proc. Nat. Acad. Sci. 98: 2515-2520, 2001.