SHH (Sonic Hedgehog) IHC Antibody

Description

The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined; however, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.

Catalog Number

IW-PA1072-1

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence near the N-terminal of human SHH, identical to the related mouse and rat sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat.Not tested in other species.

Positive Control

Brain

Cellular Localization

Cytoplasmic

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval enhance stain on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Suitable.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Tsukurov, O.; Boehmer, A.; Flynn, J.; Nicolai, J.-P.; Hamel, B. C. J.; Traill, S.; Zaleske, D.; Mankin, H. J.; Yeon, H.; Ho, C.; Tabin, C.; Seidman, J. G.; Seidman, C. : A complex bilateral polysyndactyly disease locus maps to chromosome 7q36. Nature Genet. 6: 282-286, 1994.