OPCML IHC Antibody

Product Description

Description

Opioid-binding protein/cell adhesion molecule-like (OPCML), (also called opioid-binding cell adhesion molecule (OBCAM)). OPCML is a member of the IgLON family of immunoglobulin (Ig) domain-containing glycosylphosphatidylinositol (GPI)-anchored cell adhesion molecules, as an excellent candidate for the 11q25 ovarian cancer TSG in EOC. The OPCML gene comprises 7 exons, spans approximately 600 kb, and is transcribed from telomere to centromere . And due to the lack of transmembrane domains necessary for signal transduction, it is improbable that OBCAM acts independently as an opioid receptor; more likely, it plays an important accessory role in opioid receptor function.

Catalog Number

IW-PA1131

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide mappingat the C-terminal of human OPCML, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Ovary cancer, mammary cancer.

Cellular Localization

Cytoplasmic

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin embedded tissue sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Sellar, G. C.; Watt, K. P.; Rabiasz, G. J.; Stronach, E. A.; Li, L.; Miller, E. P.; Massie, C. E.; Miller, J.; Contreras-Moreira, B.; Scott, D.; Brown, I.; Williams, A. R.; Bates, P. A.; Smyth, J. F.; Gabra, H. : OPCML at 11q25 is epigenetically inactivated and has tumor-suppressor function in epithelial ovarian cancer. Nature Genet. 34: 337-343, 2003.

2. Shark, K. B.; Lee, N. M. : Cloning, sequencing and localization to chromosome 11 of a cDNA encoding a human opioid-binding cell adhesion molecule (OBCAM). Gene 155: 213-217, 1995.