Neurotrophin-3 IHC Antibody

Description

Neurotrophin-3 (NT-3) is a member of a family of neurotrophic factors that is closely related to both nerve growth factor and brain derived neurotrophic factor. These proteins are involved in the maintenance of the adult nervous system and affect development of neurons in the embryo when it is expressed in human placenta. NT3 deficient mice generated by gene targeting display severe movement defects of the limbs.

Catalog Number

IW-PA1062

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide mapping at the middle region of human neurotrophin-3, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat.Not tested in other species.

Positive Control

Brain

Cellular Localization

Cytoplasmic

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval improve stain on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Jones, K. R.; Reichardt, L. F. : Molecular cloning of a human gene that is a member of the nerve growth factor family. Proc. Nat. Acad. Sci. 87: 8060-8064, 1990.

2. Rosenthal A., Goeddel D.V., Nguyen T., Lewis M., Shih A., Laramee G.R., Nikolics K., Winslow J.W.;"Primary structure and biological activity of a novel human neurotrophic factor.";Neuron 4:767-773(1990).