MPO (Myeloperoxidase) IHC Antibody

Product Description

Description

Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Catalog Number

IW-PA1054

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A peptide mapping at the C-terminus of NMDAR2A of human origin, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Spleen, ovary.

Cellular Localization

Cytoplasmic/nuclear

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval improve stain on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Suitable.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Klebanoff, S. J. : Myeloperoxidase. Proc. Assoc. Am. Phys. 111: 383-389, 1999.

2. Murao, S.-I.; Stevens, F. J.; Ito, A.; Huberman, E. : Myeloperoxidase: a myeloid cell nuclear antigen with DNA-binding properties. Proc. Nat. Acad. Sci. 85: 1232-1236, 1988.

3. Nauseef, W. M.; Olsson, I.; Arnljots, K. : Biosynthesis and processing of myeloperoxidase--a marker for myeloid cell differentiation. Europ. J. Haemat. 40: 97-110, 1988.