Description |
Lamins are the major components of the nuclear lamina which underlies the nuclear envelope of eukaryotic cells. lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis. Inspection of the deduced amino acid sequence of lamin B revealed the presence in coil 1B of the alpha-helical domain of a leucine heptad repeat region. Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate-bound form of the small guanosine triphosphatase Ran.
|
Catalog Number
|
IW-PA1048 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
IgG
|
Immunogen
|
A synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B,different from the related rat and mouse sequence by three amino acids.
|
Purity
|
Immunogen affinity purified
|
Conjugate
|
Unconjugated
|
Species Reactivity
|
Human, mouse, rat.Not tested in other species. |
Positive Control
|
Human mammary cancer
|
Cellular Localization
|
Cytoplasmic
|
Form
|
Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.
|
Storage
|
Store at 2-8 °C. Do not freeze.
|
Applications
|
IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Fr: Formalin or acetone fixed frozen sections. ICC: Acetone fixed cells.
|
Limitations
|
This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.
|
References
|
1. Pollard, K. M.; Chan, E. K.; Grant, B. J.; Sullivan, K. E.; Tan, E. M.; Glass, C. A. : In vitro posttranslational modification of lamin B clones from a human T-cell line. Molec. Cell. Biol. 10: 2164-2175, 1990 2. Tsai, M.-Y.; Wang, S.; Heidinger, J. M.; Shumaker, D. K.; Adam, S. A.; Goldman, R. D.; Zheng, Y. : A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311: 1887-1893, 2006.
|