Insulin IHC Antibody

Description

Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The insulin gene contains 3 exons and 2 introns; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain.Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Harper et al. (1981) and Harper and Saunders (1981) assigned the insulin gene to 11p15.5 by in situ hybridization.

Catalog Number

IW-MA1052

Quantity

9 ml

Host

Mouse

Clone

ISL-8J

Isotype

Mouse IgG1

Immunogen

Purity

Purified by the goat anti-mouse IgG affinity chromatography.

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Pancreas.

Cellular Localization

Cytoplasmic

Form

Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Formalin fixed paraffin embedded tissue sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Harper, M. E.; Ullrich, A.; Saunders, G. F. : Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Proc. Nat. Acad. Sci. 78: 4458-4460, 1981.

2. Owerbach, D.; Bell, G. I.; Rutter, W. J.; Shows, T. B. : The insulin gene is located on chromosome 11 in human. Nature 286: 82-84, 1980.

3. Huerre, C.; Gilgenkrantz, S.; Leonard, C.; Pictet, R.; Kaplan, J. C.; Junien, C. : Regional assignment of the structural gene for insulin to 11p15.1-11p15.5 by deletion mapping. (Abstract) Cytogenet. Cell Genet. 37: 495, 1984.