IHC-Tek Rat LSAB Detection Kit

The IHC-Tek Rat LSAB Detection kit is intended to use with Rat primary antibody (user-supplied) to detect the presence of antigens in tissue sections or cell preparations under light microscopy. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is also suitable for whole mount IHC staining.

Horseradish peroxidase (HRP) labeled-streptavidin and biotinylated secondary antibody amplification system has become a standard technique in immunochemical staining. IHC-Tek LSAB Rat Detection kit uses biotinylated, affinity-purified secondary antibody reacts with the user supplied rat primary antibody bound to the specific epitope of the antigen in tissue or cell. Horseradish peroxidase (HRP) labeled streptavidin then reacts with biotinylated secondary antibody to form a HRP-streptavidin-biotin complex. The HRP enzyme of the streptavidin complex catalyzed the DAB chromogen substrate (3,3’ diaminobenzidine) reaction to form brown color deposit at the antigen site. The antigen then can be visualized under microscope. Compared to traditional ABC method which uses avidin, IHC-Tek Rat LSAB Detection kit demonstrates stronger binding strength to bind biotin and less non-specific background staining.

Higher sensitivity and lower background give the kit a higher signal-noise ratio. When the Antibody Diluent is used as primary antibody dilution buffer, there is no need to perform serum protein blocking step.

Kit Components:

• Antibody Diluent: 15 ml                            
• Peroxidase Blocking Solution: 15 ml              
• Biotin Anti-Rat 2nd Ab: 15 ml                  
• Streptavidin-HRP Solution: 15 ml