Polink-2 Plus Series overcomes the weaknesses of current 2-step polymer detection systems. It demonstrates ultra sensitivity and high specificity to mouse, rabbit, goat and rat antibodies. This system reduces incubation time by 10 minutes for each incubation step, saving a total of 20-30 minutes for the entire process.
Polink-2 Plus HRP or AP Rabbit Detection Kit is an ultra sensitive polymer detection system. It uses rabbit antibody specific enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces 2-4 fold sensitivity compared to other 2-step polymer detection system on the market. User may need to further dilute primary antibody due to super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore, overcomes the non-specific staining caused by streptavidin /biotin system due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible either with manual or automated systems. Staining conditions need to be optimized by user.
1. Enhancer for Rb, 2. Polymer HRP x Rb, 3. with DAB
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