Klear Human HRP Polymer Detection System are designed for Human Primary Antibody on Human or Primate Tissue
Preclinical testing for therapy application of human or humanized antibodies includes studies such as antibody dose toxicity, hemolysis, functional lost of antibody by activation of the complement system, and an immunohistochemical cross-reactivity screen of multiple tissues. At present, greater than 99% of antibodies to human proteins are made in animal species. However, the use of animal antibody can lead to an anti-globulin response and hypersensitivity reactions. Researchers are hoping to minimize this antigenic response by developing tumor specific human host primary antibodies. Although many improvements have been made in several areas of preclinical testing, rapid immunohistochemical (IHC) screening of large sample sets have been slowed by background issues generated when screening human or humanized primary antibodies on human tissues. To improve the immunohistochemical screening process of human or humanized primary antibodies on small or large scale human tissue sets, we have developed a polymer based ready to use Klear™ Human kit. The Klear™ Human kit has an overnight priming step with the human/humanized primary antibody, followed by traditional polymer IHC-HRP/DAB application steps. We use polymer based Klear™ Human kit with primary human anti-human cytokeratin 18/19 on 9 human colon cancer and 12 human breast cancer cases to show specificity of staining. In addition, we evaluated 20 different human tissues with no primary human antibody and found this detection system produces no background.
Klear™ Human kit provides researchers with a tool to screen multiple human primary antibody clones for their sensitivity and specificity to the targeted human tumor and to assess the potential collateral damage on non targeted human tissues. In addition to Klear™ Human kit GBI already launched Klear™ Rat Kit to detect rat antibody on rat tissue and Klear™ Mouse Kit to detect mouse antibody on mouse tissue.
Simple Quick Protocol
1. Hu. Primer, 2. Quenching buffer, 3. Hu. Blocking buffer A & B, 4. anti Hu. Polymer-HRP, 5. with DAB
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