GB-Mount is a water-based mounting media for using with frozen section, immunoperoxidase and immunofluorescent procedures. AEC, AP-Red, and AP-Blue are soluble in alcohol and organic solvents. Tissue slides processed by these methods should be coverslipped with GB-Mount. Immunofluorescent assays require a mounting media that will not interfere with the assay results. When appropriate filters used, GB-Mount will not show any fluorescence background. Besides, there is no risk of quenching fluorescent emission. GB-Mount is supplied in a 18 ml a dropper bottle or 100ml bottle.
Recommended Protocol: 1. After saline rinse, shake off excess liquid from the slide and quickly wipe the back of the slide. 2. Invert dropper bottle to displace bubbles from the tip-end. 3. Squeeze out the first drop onto a paper towel to remove of air bubble on the tip. 4. Add a few drops of GB-Mount on the tissue section on the slide. 5. Carefully lay down coverglass to cover the tissue section. 6. Gently press out any noticeable air bubbles. If there are many bubbles on the slide, one may soak the slide in saline, remove the coverslip, and repeat the coverslipping procedure 7. The slide may be viewed immediately after coverslipping, be careful not to dislodge the coverslip before the slide has dried. 8. Allow sufficient time of drying slides before storage. 9. For maximum retention of fluorescence, slides should be kept in dark place at 4°C.
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